📍 Collaborative Prototyping Core Facility, University Hospital Bonn, Germany
🔬 Multiple Custom-Built Multi-Photon Microscopes by Independent NeuroScience Services (INSS) and Nikon A1R+ with Confocal Unit
- Maintenance, trouble shooting and repairs of imaging systems at the Institute for Experimental Epileptology and Cognition Research (IEECR) and Institute of Physiology II.
- Development, assembly, programming and integration of devices for sensory stimulation and behavioral monitoring.
- Laser coupling, alignment and modification of optical systems.
- Calibration with slides (pollen, grids, beads and illumination).
- Scheduling meetings with service partners for further refinement of imaging systems.
📍 German Center for Neurodegenerative Disease (DZNE) in Bonn, Germany
🔬 LaVision TRIM ScopeII with OPO
- Development, assembly, programming and integration of devices for sensory stimulation and behavioral monitoring for in-vivo imaging in mice.
- Deep brain GRIN-lens volume calcium imaging in awake mice.
- Computational analysis of calcium imaging data (suite2p, CaImAn, CNMF-E, clustering).
- Extraction of imaged tissue for EASI-FISH.
🔬 Axio Zoom.V16 and AxioScan.Z1
- Batch scanning of viral injection sites for anatomical reconstruction of infected neurons.
🔬 Zeiss LSM900 AiryScan
- Recording of multi-channel FISH probes for a variety of markers in mouse brain slices.
📍 Technical University of Kaiserslautern (formerly TUKL, now RPTU), Germany
🔬 Bruker Ultima Investigator Multi-Photon Microscope
- Development of olfactometer (with GUI), optogenetics harware, feeding system and integration in existing imaging setup.
- In vivo imaging of engram cells and Mushroom Body neurons with olfactory stimulation and optogenetic activation in Drosophila adult brains in.
- Recursive image batch processing with Fiji macros (.tif extraction, registration, segmentation, background subtraction, trace extraction, data structuring).
- Analysis and plotting of extracted calcium traces with MATLAB (import of data structure, protocol correlation, statistical testing of neuronal activity, correlation and hierarchical clustering of neuronal activity, amplitude, area under curve).
🔬 Leica Stellaris tau-STED and Laser Scanning Confocal Microscope
- Chemofluorescence (chemical tags) and z-stack acquisition of synaptic markers in engram cells of Drosophila adult brains.
🔬 Leica TCS SPE Laser Scanning Confocal Microscope
- Immunofluorescence and visual quantification of engram cells in Drosophila adult brains.
🔬 Zeiss LSM 700 Laser Scanning Confocal Microscope
- Immunofluorescence and z-stack acquisition of mushroom body neurons in Drosophila adult brains.
📍 Charité Institute for Neurophysiology in Berlin, Germany
🔬 Leica SP5 and SP8 Laser Scanning Confocal Microscopes
- Immunofluorescence, genetic labeling and z-stack acquisition of specific neurons and synaptic markers in Drosophila* adult brains*.
🔬 Leica DMI 6000 Inverted STED Microscope
- Immunofluorescence of synaptic active zones in Drosophila larval NMJs.
- STED image acquisition of Active Zones in larval NMJs in Drosophila.
- Recursive image batch processing with Fiji macros (STED ring-diameter measurement).
🔬 Femtonics Femto2D Resonant Multi-Photon Microscope
- Implementation of olfactory stimulation, feeding, optogenetic LEDs, electrophysiology, IR camera for behavior monitoring via NI-DAQ and LabView.
- In vivo calcium imaging with olfactory stimulation of GCaMP-expressing mushroom body neurons in Drosophila.
🔬 Nikon A1R+ Multi-Photon Microscope with Confocal Unit
- Implementation of olfactory stimulation, optogenetics and picoinjection via NI-DAQ and Nikon Imaging Suite (NIS) Elements.
- In vivo calcium imaging and olfactory stimulation of GCaMP-expressing mushroom body neurons in Drosophila.
📍 Friedrich Miescher Institut (FMI) in Basel, Switzerland
🔬 Leica TCS SPE Laser Scanning Confocal Microscope
- Immunofluorescence and visual quantification of synaptic markers in larval neuromuscular junctions (NMJs) in Drosophila.
🔬 Zeiss LSM700/710 Laser Scanning Confocal Microscopes
- Immunofluorescence and z-stack image acquisition of synaptic markers in larval NMJs in Drosophila.
🔬 Zeiss lightsheet Z.1 microscope
- Evaluation for trans-cuticle in vivo imaging of larval NMJs in Drosophila during development.
🔬 Olympus IX81 with Yokogawa CSU-X1 Scanhead Inverted Multipoint Confocal Spinning Disc Microscope
- Genetic labeling of synaptic markers with fluorescent proteins in the Drosophila NMJ for in vivo trans-cuticle imaging during development.
- Image deconvolution with Huygens Software.
- Recursive image batch processing with Fiji macros (maximum intensity projections, alignment, montages).